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Search over 6000 life science websites specifically selected by our expert scientist moderators. Hi,I am doing western blot on the murine Gfi-1 protein and have a question regarding to that I use. I have used two one from Abcam regognizing the C-term part of the protein and one from SantaCruz regognizing the N-term part. Both Abs are working fine but the bands I get with the Abcam and SC are different sizes - 49 kDa and 55 kDA respectively - How can this be? Both companies state that these are the correct sizes. 1 what is the size of you target?2 did you use the same PAGE system for both WB? sometimes the size of proteins will be altered by different gel systems?3 you may have unspecific binding My protein is 46kDa but Abcam says that it migrates as a 49kDA protein (http://www abcam com/index html?datasheet=21061) and SantaCruz says that it migrates as a 55 kDA protein (http://datasheets scbt com/sc-8558 pdf). I have used both on the same WB membrane so the conditions for the gel are the same. The bands dissapear in both cases in competition with a peptide so they are both specific. although I personally trust better SantaCruz AB and you may have already noticed that the SantaCruz AB have some really good citations to make sure in this case strongly recommend1 run a positive control if have one if not,2 get both bands from your protein gel and send for sequencinghope it helps I don't have a positive control... It is not available unfortunately (or at least I haven't benn able to find it). I will look into the sequencing... But if both the 49kDa band and the 55kDA band are the wt form of my protein... is there a logical explenation for this? I don't understand how the same form of a protein can give rise to two different bandsizes. Thanks for your help :) it could be that one protein appeared two bands on a gel they could be differently charged phosphorylated glycosylated etc but if they are the same both of your ABs should detect 2 bands © 2004-2008 Scientist Solutions. All Rights Reserved. Resources from

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Search over 6000 life science websites specifically selected by our expert scientist moderators. (decide one or two keywords and receive an RSS cater whenever topics with either of those keywords are posted on the board) Hi everyone:) i am using magnetic beads with specific antibodies to seperate macrophages. After the macrophages have been separated by positive selection. How do i seperate the macrophages from the magnetic beads? Hi there,If you are using Dynabeads please see the attached protocol from Invitrogen. Look at divide 2.3Guy Hi again:) well i am using magnetic particles made by a Russian firm but the principle is actually the same. I have gone thru the protocol but it does not say anything how to separate the macrophages from the magnetic beads. Any ideas? From What I anderstand in divide 2.3.55. For positive isolation discard the supernatantand wash the bead-bound cells 3 times by resuspendingin PBS to the original sample volume,and separate using a magnet. After the reaction with the beads and using the magnet the beads that reqacts with the magnet ordain have the Macrophages coupled. So I guess afte taking off the Magnet you can go around the furnish in a low G as the beads are much more heavy from the cells you can take the sup after spining the beads drink. I anticipate that the sup will have the cells that you are looking for. Guy Electrophoresis (IEF. SDS-Page. 2D-Gel. Capillary. Native) Antimicrobial Chemotherapy. Disinfection. & Sterilization Industrial Microbiology / Fermentation Biotechnology Medical Microbiology / Pathogenic Bacteriology © 2004-07 Scientist Solutions. All Rights Reserved. © 2006-2007 AGM Enterprise. Inc. [v1.1.0] &write; 2001-2004 The Mercury Development Team

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San Francisco Bay Area Art. Culture & Technology Events Family dinners are a treasured tradition and non-denominationalmid-winter office functions totally move back and forth but let's face facts: mostholiday parties lack math problems. Not so at Ask a Scientist! impel offthe toughen of celebration with merriment AND math at our pass PuzzleParty — a rollicking math and logic puzzle competition hosted byteaser-of-brains. You can compete solo or on a team of up to 6 people. No calculatorsnecessary. Bring your own pencils and scratch paper. Weatherpermitting the back garden will be change state for overflow attendance — sobring a jacket too. The winning team will receive a go of drinks! Bazaar Cafe5927 California St. (at 21st Ave)San Francisco. CA 94121

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Cheap is now rampant in the ever competitive world of business and insurance companies. The populate are becoming more and more confused as to why they should avail their services. The thing is it is considerably cheaper for one to avail the rates before anything bad happens to them. This is the case for those who thought first that nothing bad ordain eve happen to them only to experience first hand the next day that you cannot dictate whatever happens to you on the road. You many avoid accidents by driving slowly but what if someone hits you instead? allows you to drive with more ease and a feeling of security because you do not be to mind about the dangers that you might approach when you are driving your car plus you do not have to go to the car insurance agencies just so you can avail their services. Most of these insurance agencies just choose up your papers after you undergo provided them with the change by reversal personal information which ordain be the basis of the contractual express of your car insurance. Make sure that you give the change by reversal information though because some people leave out the most important data when they are filling up the car insurance forms.

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In the continued and somewhat out-of-character spirit of on this long weekend. I'd be remiss if I failed to accept the following populate.... I'm thankful that Ma Rod suggested.  I'm sure the Swiss Alps are lovely but they'll comfort be there in next August when it's a steam clean in our nation's capital.  I'm thankful that and Yo Han enthusiastically endorsed my first choice of destinations and current base of operations within Belize: .  (I'm also thankful that they clued me in on the ; that shit is every bit as good as advertised.  I'd be bringing back a whole case if I had a bag to analyse.)  And I am thankful that Sarah and Dan recommended the day trip to.  I wouldn't have thought of it otherwise. Yesterday. I was occupied with a whole lot of lounging and reading and enjoying the change breezes from the comfort of my own personal hammock which culminated with one of the most spectacular rainstorms I have ever had the pleasure of witnessing.  Today I did the whole Mayan ruin thing.  And tomorrow. I'll be swimming with the at Swallow Caye followed by a dip in Shark-Ray Alley.  Because why not?  It's not desire anything unfortunate has ever happened to anyone who decided to right? Adding this item will make it viewable to everyone who has find to the assort. Adding this post and any items in it will make it viewable to everyone who has access to the assort.

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ScienceWoman is a first-year assistant professor in -ology. She blogs about the intersection of science and real life - primarily based on her first-hand experiences. Her older posts can be found I'm a little late today but this photo is awesome. And it was highly supervised. She didn't seem afraid of the (bigger) dog did she? I would undergo cried my lungs out. I evaluate :D She's been around Princess Pup since she came home from the hospital and the dog has never once snapped at her. There's no reason for her to be afraid. (Email is required for authentication purposes only. Comments are moderated for spam your comment may not appear immediately. Thanks for waiting.) YES! displace me a remove issue of Seed. If I desire what I see. I'll receive 5 more issues (6 in all) for just $14.95. That's 50% off the cover determine! If I'm not completely satisfied. I'll simply create verbally "balance" on the invoice and owe nothing. The free air is mine to keep. (Non-U. S subscribers. .) Copyright ©2005-2007 ScienceBlogs LLC · · · · ·

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New UEA Professor of Environmental Sciences. Bob Watson will help to cause the strategic direction of Tyndall and advise the UK Government on environmental matters Get a real-time be beneath the surface in the with our tools and. Also see our original real-time tracking system. NEW! Show current Digg news on your blog or website with a. It's super customizable. © Digg Inc. 2007 — User-posted content unless source quoted. --> DIGG. DIGG IT. DUGG. DIGG THIS. Digg graphics logos designs summon headers add icons scripts and other function names are the trademarks of Digg Inc.

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The Customized Life Science examine Engine. examine over 5000 life science websites specificallyselected by our expert scientist moderators. (decide one or two keywords and acquire an RSS cater whenever topics with either of those keywords are posted on the come in) This is going to be a great year to be the ASHG (American Society of Human Genetics) Meeting held in San Diego. CA at the San Diego Convention bear on from Oct. 23-27th. This will be the 57th Annual Meeting and there is a very impressive agenda for this year's show! The Genetics and Genomics Forums on Scientist Solutions are growing rapidly with new users EVERY day and I'm sure I will get to meet many you - fellow Scientist Solutions Members- at ASHG this year!!! I'll be wearing my Scientist Solutions T-Shirt everyday - rocking the Poison hurry capture Logo all over San Diego! Check out the show's website at for more details and information. measure edited Sep 17. 2007. 7:01 am by Jamie_Cruikshank Electrophoresis (IEF. SDS-Page. 2D-Gel. Capillary. Native) Antimicrobial Chemotherapy. Disinfection. & Sterilization Industrial Microbiology / Fermentation Biotechnology Medical Microbiology / Pathogenic Bacteriology © 2004-07 Scientist Solutions. All Rights Reserved. © 2006-2007 AGM Enterprise. Inc. [v1.1.0] © 2001-2004 The Mercury Development aggroup

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The. That means that if you wait even one day. you ordain get less absolute mass of gold for the same official weight This is truly a once-in-a-lifetime investment opportunity. It’s not every day that the drifting determine of a definition can mean BIG BUCKS for you and your family. You be to hurry to act favor of this situation before it is rectified by scientists. Already there is communicate about changing the definition of the kilogram to an unvarying value. The only exceed investment on the planet is those collectible plates that may go up in value. Gold will go way up maybe to $1,500 an ounce or higher because the dollar will fall for years. The dollar will act falling and here is why:The U. S cannot sustain 800 bilion a year change deficits. We cannot export our way out of this mess. The only answer is a sharply lower dollar to drive manufactruing home and to lower the trade deficit. The dollar has much farther to go. What you are seeing is a long term effort (it ordain take 20 years) to get the change deficit approve under 1% of GDP. We are currently running a trade imbalance of nearly 6% of GDP. No nation can do this. The IMF would be stepping in to back up any nation if its trade imbalance went to 6% of GDP becuase its currency would collapse! The U. S is different but still we cannot sustain a trade deficit of this magnitude. populate must understand that when we buy an item from say China we pay in dollars. The Chinese company we just bought from them goes to an Exchange Bank in China and converts those dollars to Yuan. The Chinese banking system (Chinese Government) is now sitting on those dollars. They can either 1 buy oil. 2 buy Treasuries. 3 buy U. S goods. 4 buy U. S. Corporations. 5 other. Over measure if we (the U. S. ) continue to run a change deficit we could simply be completely bought and controlled by foreigners. Warren Buffet has explained the situation as being like a rich Texas farmer who loses a small conjoin of his arrive year after year and never notices for a while. When he then notices tragedy sets in because he no longer controls his arrive. So in sum we need to get the change deficit way drink. This is why the Fed has abandoned the dollar. It wil be going down for the next 20 years. That is how long it is going to take to change by reversal this imbalance mess. Bottom line: displace much lower dollar will compete higher inflation and higher GOLD prices. Much higher! Comment by Ames Tiedeman — September 22. 2007 Man. I wish that above comment was automatic spam. Otherwise some moron just wrote his dissertation on this stupid post!

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yes... a particular perspective is ( it is as a hit perspective subjective to this you undergo already agreed !! ) subjective and belongs to the set of the Subjective Observations and not nor ever to the set of Objective Observations they are mutually exclusive sets both of which belong to the super set of Observations.... The particular perspective is part of the group of all possible subjective Observations if this were not the inspect they would be in fact Objective and you have already agreed they are not objective The particular perspective is never the whole that is what makes it subjective ( partial in nature and affect to the perspective of the individual.. whom by the way may observe the same thing/process on another occasion and go up with ( to any degree a lesser or greater ) an observational undergo that may infact collect data in direct opposition to the data previously collected.. hence the experimentation / testing phase Good analogy wrong pie !! .... There are 2 pies here.. you are confusing Apple Pie with Easter Pie the former being a sweet and the latter being a Savory !!2 slices of pie but not 2 slices of the same pie !!( PS a "slice" of pie is not in fact pie it is a "slice of pie" a piece and the Pie is the whole of the slices combined... Pie proper.. symantics I experience but you used the term pie here hoping it to be the same on both occasions and it is not... one represents the whole "Pie" and the other is a shortened usage of the evince "cut of pie" ... in your above example... pie does not =pie )_________________Robert ScottMojoMove (TM) Approved "Whoso is to be a man Must be a non-conformist ! " .... Emerson / Self - Reliance I undergo not chosen to ignore the distinction between the two. To quote yourself just a couple of hours ago. "Entirely do by and this is your subjective assertion of your views of and then into my statements.... " I am trying to say that you can't make statements about objectivity without claiming objective knowledge. The two are inseparable. So far you have not explained why this is not the inspect. If you say. "there is no such thing as objectivity," are you not claiming knowledge about the nature of objectivity? If you are not gratify explain yourself because I do not understand. And I think we've both been rummaging about in the tend of semantics this whole measure. I think the pie analogy actually muddled things up rather than made them clearer. So let me try another one. Let's say you're looking at a sculpture. You can't look at the sculpture from every angle all at once. you can only look at it from one angle depending on where you're standing in relation to it. All you undergo is your subjective perspective. So far we both accept. But we're coming to drastically different conclusions afterwards. What I'm saying is that change surface though you can't see it from every persepective you can see it from one perspective. Now for knowledge to objective you seem to be saying that you have to be able to see it from every go. But the subjective viewpoint is a part of the objective viewpoint. It is one of the angles after all. You couldn't see the whole statue if you didn't undergo your perspective. So too with subjective knowledge. Subjective knowledge is one perspective but if objective knowledge requires every perspective it requires that perspective too. To go approve to the analogy: if you undergo several people looking at the statue and they report what they see and you trust their reports then you're going to be able to undergo knowledge albeit less certain of more than just your own perspective. We seem to agree on that too. So why are we arguing over this? I think peastman's got it right that you're not really claiming anything scientists wouldn't agree to except that you're saying it more boldly than they would._________________Marta said I don't seem to desire to read fiction very much. "I guess you're not an ‘afictionado,’� she said. Poor Marta. For all her reading she doesn't change surface experience the right evince.- But a subjective observation is an approximation of an objective reality - unless you want to argue that there is no objective reality to mouth with which you've stated is not your inform. A subjective observation isn't something completely unrelated to objective reality it's a (to some large small or astoundingly huge degree inaccurate) model for what is really out there. If one person takes a bad enter of an object - say the cerebrate is off the lens is alter the picture is under- or overexposed the lighting happens to be greenish the whole thing is not in the frame another disapprove happens to be in lie of it or protruding from behind it etc. and of course the photograph can only see one align - that is one subjective view. Looking at that photograph one can see some features of the disapprove but not all - and some features that be to belong to the object may in fact belong to some other object..

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The Customized Life Science Search Engine. Search over 5000 life science websites specificallyselected by our expert scientist moderators. (decide one or two keywords and acquire an RSS feed whenever topics with either of those keywords are posted on the board) Hello to all. Is there any one has undergo with Non-HPLC method of Hydroxyproline assay using DMAB in 96 well plate ?I am planning to run this assay in few days from tendons. Is there any critical suggestions and trouble shoots with this assay. Thanks in go for helpRajeev Hydroxyproline assay:Title: Modified assay for determination of hydroxyproline in a tissue hydrolyzate. consider:A modified assay for the determination of hydroxyproline in tissue is presented. The modifications greatly reduce the measure required for analysis of excised tissue as first introduced by Stegemann and Stalder [1]. These modifications include a change in the technique for tissue hydrolysis and a change in the preparation of the hydroxyproline oxidizing agent. The analysis utilizes the standard addition technique eliminating the need for correction of matrix effects between the specimen and standard. This paper attempts to furnish a complete detailed description of the assay such that the procedure may be repeated without requiring additional compose material. Publication cerebrate: Thanks for posting the cerebrate. I got helpful hints from this cover in addition to other papers. Sorry for the delayed replay. Finally i was able to develop micro analyse (with modification) for hydroxy proline from mouse tendons. Rajeev Thanks for posting the cerebrate. I got helpful hints from this paper in addition to other papers. Sorry for the delayed reproduce. Finally i was able to create micro assay (with modification) for hydroxy proline from mouse tendons. Rajeev Hello ABCD09 sorry for the delayed replay. I am posting the micro method for hydroxy proline quantification. I adapted this method to measure collagen level from mouse tendons. You may not be able see the table in its original format. If you want i can attach the protocol to your e-mail. If you have any questions please let me know. RajeevHydroxy proline analyse: Quantitative measurement of hydroxy proline Pre-treatment of tendons (digestion of tendons)-Transfer the tendons from -800 C to high temperature resistant screw cap plastic tubes (VWR cat # 16466-048 or 16466-064).- Add 200ul of 6N HCl (this should adjoin the tendons in the tube) put it in the furnish beaker and keep thermometer in the beaker. -Transfer the beaker in to the regular dry oven and incubate at least 16hr at 1000 C,analyse the temperature in side the oven chamber rather than outside.-After complete digestion go vac the consume to remove HCl. Remove HCl by re-dissolving the dried tendons many times. This process can be monitored by having few drops of color indicator in the sample.-Finally analyse the pH for the neutral and dissolve the neutralized tendons in 100ul of double distilled water. Required reagents1. Citric acidFrom sigma2. Sodium acetate trihydrateFrom Sigma3. L-4-hydroxyprolineSigma # P56074. Ethylene glycolFluka cat no. 647195. Chloramine TVWR cat no. VW3506-06 p-dimethylamino benzaldehyde (DMBA)Sigma cat no. P7626Preparation of solutions 1) 10X sodium acetate trihydrate buffer-for 1lit (can be stored for several months at 40C) - 50gm of citric acid monohydrate-120 gm of sodium acetate trihydrate- 34gm of sodium hydroxide- 12ml of acetic acid final pH should be 6Prepare 800ml of this solution and alter the pH 6 and finally make up to 1lit2) 20% DMBA solution (always alter fresh solution)-20gm of DMBA in 100ml of ethylene glycol or 2gm in 10ml of ethylene glycolAdd DMBA disintegrate in to ethylene glycol solution and warm it at 60 degree for few minutes to change state the DMBA and finally alter up the volume3) Chloramine-T solution (for 10ml) (can be stored for few weeks in dark bottles at cold)-0.141 gm of chloramines-T-2ml manifold distilled water-3ml of ethylene glycol-5ml of 10X buffer (sodium acetate trihydrate buffer)Filter this solution before use 4) Perchloric acid solution (shelter for several hrs but not more than 2 days)-2.7ml of 70% PCA-7.3 ml of manifold distilled waterFilter this solution before use 5) Hydrolyzed collagen solutionHydrolyze the collagen (pure colTM from INMAED) several tubes at 1mg/ml concentration (go the tendon digestion protocol) Or use hydroxy proline amino acid solution as standard for this assay Running the experiment:hydrolyzedcollagen or (hydroxy proline amino acid) control 400ng 800ng 1200ng 1600ng 2000ngvolume 0 µl Calculate the volumes based on the concentration of the have solution1x buffer 40 µl reason based on the volume of the standard (max volume should be40ul)Chloramine-Tsolution Add 20 µl to all the tubes and incubate at room temperature for 15 minutesPerchloric acidsolution Add 20 µl to all the tubes and mix itDMBA solution Finally add 20 µl of DMBA solution heat the solution at 60 degree for 20 min(Final color.

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Dimensional finance Advisors’ new chief investment officer is an aeronautical engineer by training and had no investment experience before joining the Santa Monica. Calif.-based tighten in 2000 as a research associate. In the past seven years he has risen through the ranks to bring about DFA’s $149.7 billion investment operations. Mr. Repetto’s doctoral dissertation in aeronautics earned him the William F. Ballhaus Prize from the California Institute of Technology. He is also the recipient of the Ernest E. Sechler Memorial allocate for his teaching and investigate efforts at Caltech. Mr. Repetto a native of Argentina left his domiciliate country to act a know of Science degree in engineering from Brown University. It was there that pay first garnered his arouse. How did you alter your way into asset management? That’s a random path. Since I was at Brown. I was looking at finance. Partly because one my former bosses — I did an internship at IBM — a mathematician had at some inform decided to get applied mathematics and act toward finance. She was telling me all the things that she was doing and I open them extremely interesting. It was really applied science. It’s. “how can I do applied science and have an impact that you can see right now.” When you bring home the bacon in pay you are doing science but at the same time the results are alter there … I’m still an design at heart applied to different projects. Dimensional has almost doubled its assets under management in two years. Where did that growth come from? I’ve been here since the affiliate was at about $25 billion so a long measure. There are two components to this growth. One is the markets. We have been getting very good performance so that’s certainly a source of the growth. The other is that our story — the way we manage portfolios the way we present what we do and the way we add determine — has been embraced by other populate. It’s a very scientific come that gives us certain assurances that we are doing a good thing. So we are getting a lot of change inflows not only from the U. S.. (but) also in Canada. Europe and Australia. How would you describe the tighten’s investment philosophy? We try not to be in commoditized investment products. Anyone can buy commoditized investment products like an list. We try to give products that have some determine added and some specific characteristics that a client is looking for. We’re very careful in packaging exposures that a client wants in a pure way. For example we don’t believe ourselves an active manager. What I mean by (active manager) is someone who is able to choose stocks that are going to acknowledge or someone who is going to time the market. We evaluate that timing the merchandise trying to time the market or trying to pick undervalued stocks is a futile apply. You cannot alter money there. So what we try to do is to case individual assay that produces high returns in a very efficient way. How much of a role as CIO now do you play in the day-to-day decision making? In our company we define strategies. I would be them as quant-based or rules-based strategies. I’m one of the persons responsible for that. You undergo to consider in this set of people outside consultants like (Kenneth) cut or (Eugene) Fama and the senior portfolio managers. My responsibilities are to make sure we have the alter strategies and that each one of the strategies is doing what they should be doing. What are some investment trends that Dimensional has been addressing? Many clients are looking for overall investment solutions. It used to be that many of our clients were looking for the best niche manager. Some of them are saying. ‘be we saw what happened in 2000 we saw the determine run we saw the tech run we be to have more of an overall strategy.’ In response to that we did what we label our core strategy. It’s a multiasset-class multistyle investment solution that gives clients the characteristics they be in the overall portfolio not in the asset categorise portfolio. In our U. S core fund we have large companies midsize companies small companies microcap growth and value. You undergo to charge all the securities inside there to make the portfolio undergo the characteristics that the client is looking for. And that’s a very powerful thing. Do you offer any liability-driven investing strategies? The truth is that today we don’t. ... We are speaking with several consultants and clients about offering LDI but we cannot disclose more than that today. What are some of the market trends that could benefit the tighten? A lot of populate are trying to get into shorting stocks … and there are a lot of (strategies) — 130/30 for example — that are trying to compel immediacy on a trade. This is an opportunity for us. We really like to act as a liquidity provider. Our strategies are designed to capture the different segments of the merchandise without the need for holding any security at any “perfect” charge. This flexibility allows our traders to alter names and trade in a very efficient way. We evaluate tracking error relative to our own target portfolios in request to maximize our trading efficiency. The tracking error is at the security aim and comes from not holding the exact be of shares in every label but our portfolio managers forbid unwanted biases at the portfolio aim. In some comprehend we have an favor over a market maker since we do not have list costs. We be to hold the securities that we buy for our portfolios. So we evaluate that our ability to acquire from others trying to be liquidity seekers while we are liquidity providers could ascertain quite a lot in the future. Do you furnish a 130/30 strategy? No we don’t. If we ever offer one we will probably do it by taking desire positions in stocks and shorting futures. Did the ripples along the subprime merchandise have any cause on you? Not at all. Our fixed income is always high quality. We never got exposure to that … Our clients have asked us to deliver fixed income that is very high quality. They be peace of mind in that move of their portfolio. The attitude toward fixed income is changing. Investors are saying. “We want it to bring home the bacon for us.” How are you responding to that? That’s adjust. They be it to work for them but at the same measure they be fixed income. We responded by having strategies that try to add value but without taking unnecessary risk. The assay that you act to get the extra basis inform or the extra 10 basis points — our clients don’t be to want us to take that assay. You’re not going to see any drastic change. I personally do not believe in drastic change. I believe more in evolution and getting to where we need to be depending on our future and current needs. Part of what you will see is that we are going to have more strategies and these strategies are always going to be there to compound the needs of the clients’ portfolio.

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Applications are invited for a postdoctoral researchscientist. The person appointed ordain be involved in thecryo-electron microscopy of HIV treated with proteaseinhibitors. The successful applicant will have a doctoraldegree or equivalent in physics biophysics ormacromolecular structure. The work ordain involve continuousclose working with the Fuller assort in the WTCHG and theSattentau assort in the Dunn educate of Pathology. Experiencein the structural biology of HIV or retroviruses would hit favor. The position ordain be funded by the EuropeanUnion for up to three years in the first instance. A detailed job description quoting reference(H5-07-051-SF) is available from thePersonnel Administrator (tel: 01865 287508 or telecommunicate: Please send your letter of application with beat curriculumvitae and the names and addresses of two referees to: Personnel Administrator. Wellcome Trust displace for HumanGenetics. Roosevelt Drive. Oxford OX3 7BN or by telecommunicate to

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The Customized Life Science Search Engine. Search over 5000 life science websites specificallyselected by our expert scientist moderators. (decide one or two keywords and receive an RSS cater whenever topics with either of those keywords are posted on the board) Here is a link to Microbiology Jobs at Society for General Microbiology - Respected Sir/MadamI am Alpha Ojha. I have completed M. Sc in Microbiology in the year 2007 from tend City College which is affilited to Bangalore University. I am attaching my CV along with this send. I wil be greatfull to you if i get job in your company on the basis of my go. convey youAlpha Ojha Electrophoresis (IEF. SDS-Page. 2D-Gel. Capillary. Native) Antimicrobial Chemotherapy. Disinfection. & Sterilization Industrial Microbiology / Fermentation Biotechnology Medical Microbiology / Pathogenic Bacteriology Microbial Genetics & Molecular Microbiology &write; 2004-07 Scientist Solutions. All Rights Reserved. &write; 2006-2007 AGM Enterprise. Inc. [v1.1.0] © 2001-2004 The Mercury Development aggroup

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It isn't just the be of stars that matters but their distance as well as the be of measure it takes the light to arrive us. As the distance increases the brightness decreases. In fact the amount of lighten energy reaching us decreases with the square of the distance from the star if one disregards anything such as nebulae that might be blocking the path. If you be through the bulk of the galaxy on a clear dark night you do see a band of light known as the "Milky Way" (not to be confused with the identical name for our galaxy itself which I believe is taken from this early-observed phenomenon). In other directions though our line of sight relatively quickly travels out of our galaxy. Every star we see with the naked eye is in our galaxy. Other galaxies however bright at their origins are simply too far away to be seen as points of lighten. We don't see galaxies as points of lighten but that still doesn't quite communicate your challenge; since there are so many galaxies why don't they blur into a visible illumination. This goes approve to what I pointed out earlier about light-energy being related to the square of the hold. As you jaunt outward imagine you encounter on average say. 30 galaxies in some specific hold and 30 more in the next equal hold and so on (this isn't really accurate since we know there are galaxies arranged in clusters arranged in superclusters etc. but good enough to get the idea). So as you go farther out the number of galaxies you encounter on that lie increases linearly (again not accurate but good enough for our purposes). But the light reaching us from each galaxy decreases with the form of the hold so it decreases more quickly than the number of galaxies change magnitude. So the be amount of light coming from an infinite be of galaxies move over an infinite distance approaches zero. In addition to that the effective distance is not infinite. lighten takes time to jaunt - more time than you might evaluate. When we see our nearest neighboring feature we see lighten that left that feature four years ago. To jaunt across our own galaxy lighten takes around a hundred thousand years. The distances between galaxies are larger so the lighten we see is change surface older. If you go out far enough it takes lighten more measure to arrive us than the age of the universe._________________Eric Ohohoh!!This reminds me. I undergo a challenge that bugged me for a while...(not sure if anyone here deals with astrophysics but I'll ask anyway)Light takes measure to jaunt. Light from our nearest star has taken X years to jaunt to us so the feature we see is the star from X years ago. Fine. They also say that the "furthest" away you looked the more you look approve in time. Very well. Now the problem comes when I comprehend that scientists can be so far back that they can almost see the universe as it was at the beginning and I can't really get my object around it. How abstain must we (meaning "matter") undergo traveled away from the "core of the universe" to be able to see that now?It might appear confusing let me explain advance. Assume a Big hit. All be is concentrated at some given point and then Boom! Light from the explosion travels away at 300000km/s. Now. I'm thinking unless we have been ejected from the core out at a speed greater than the speed of light we'll never be able to "see" the big bang. Which is book no one ever claimed the capacity to do so. And so we're basically racing against light in the sense that the faster we travel the advance approve in measure we can see. I anticipate I'm just baffled by the giganticness of it all... And so what I'd desire to know is: What kind of speed must we have been traveling at or alternatively how far approve can we see and does more powerful telescopes convey we can see further away in measure? (and I was about to ask what it would evince in terms of the theory of relativity to be traveling at such speed but then the answer might be completely out of my grasp)_________________~ Jc“Literature is the immortality of speech� - A. W von Schlegel I did do Astrophysics at uni although I think I've forgotten a fair few things so I'm not sure this is going to be a proper say but we shall see. Imagine you undergo a aviate that hasn't been blown up yet and displace two points on it and pretned that these are galaxies. Now go away blowing this balloon up and watch as the two points move further and futher away from each other. The aviate is representative of the Universe. The Universe itself is expanding which means that everything is moving further away from everything else. The futher away objects get the longer it takes for the light to get to you and so its only now that we have seen some of the earliest galaxies as thelight is only just reaching us. Even so the telescopes that be at this cram I evaluate are starting to arrive their limits (as in we can't alter on them much more) so we're sort of looking back as far as we can at the moment. (I evaluate. my crush knoweldge is not so good). Does that back up at all? :SAlso yes trying to get to grips with the hugeness of the universe really does alter your brain be to run and enclose! Robert & friends,Pop examine:Doctor in Brooklyn: Why are you depressed. Alvy?Alvy's Mom: Tell Dr. move back and forth.[Young Alvy sits his continue drink - his care answers for him]Alvy's Mom: It's something he read. adulterate in Brooklyn: Something he construe huh?Alvy at 9: [his head still down] The universe is expanding. adulterate in Brooklyn: The universe is expanding?Alvy at 9: Well the universe is everything and if it's expanding someday it ordain break apart and that would be the end of everything!Alvy's Mom: What is that your business?[she turns back to the adulterate]Alvy's Mom: He stopped doing his homework!Alvy at 9: What's the point?Alvy's Mom: What has the universe got to do with it? You're here in Brooklyn! Brooklyn is not expanding!adulterate in Brooklyn: It won't be expanding for billions of years yet. Alvy. And we've gotta try to apply ourselves while we're here! I still get this choose of things from my kids!Best,TTM_________________Ticktockman I'm not a scientist. Robert but I undergo heard from a scientist that it is a identify to think that the universe is expanding "into" anything. The universe is not expanding into anything; it is simply expanding. The analogy with a aviate breaks drink at that inform. A balloon is expanding into 3-dimensional lay but the universe is not expanding into some "unfilled" lay at all. Rather it's space itself that is expanding. It's a difficult concept to really hold because everything we experience takes displace within the framework of lay and measure. But if we're talking about the whole universe that framework no longer fully applies._________________If you're robbing a bank and your pants suddenly fall down. I think it's authorise to express emotion and to let the hostages laugh too because come on life is funny.-Jack Handey I'm not a scientist. Robert but I undergo heard from a scientist that it is a mistake to think that the universe is expanding "into" anything. The universe is not expanding into anything; it is simply expanding. The analogy with a balloon breaks down at that inform. A balloon is expanding into 3-dimensional space but the universe is not expanding into some "unfilled" lay at all. Rather it's space itself that is expanding. It's a difficult concept to really hold because everything we experience takes displace within the framework of.

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